Plasmid Technology

Plasmid isolation. Fatemeh Mehrpooyan*

Plasmid Curing Activity of Five Plant Extracts on Multiple Resistant Plasmid Bearing Klebsiella Pnumoniae Strains

ABSTRACT The curing of five medicinal plant extracts (Satureia hortensis, Glycyrrhiza glabra, Teucrium Pollium, Myrtus Communis, Eukalypetus globolus) on plasmid bearing strains of klebsilla pnenmoniae resistance to multiple antibiotics were investigated. Alcoholic and maceration extraction were carried out from leaves, fruits or roots of the above plants. Mimimum inhibitory concentrations (MI...

Plasmid-to-plasmid recombination in Haemophilus influenzae.

No recombination between plasmids was observed after conjugal transfer of a plasmid into a cell carrying another plasmid. Two types of such recombination took place after transformation, one type being Rec+ dependent and suggesting a preferred site of recombination. The other much rarer type was at least partially Rec+ independent.

Plasmid Purification

This paper describes a new method o5 plasmid DNA purification which is fast and reliable enough for most purposes in recombinant DNA technology. The present method does not require the use of toxic chemicals such as phenol or e th id ium bromide, costly ultracentri /ugation procedures or other processes which can modify the supercoiled structure of the plasmids, such as adsorption on glass fibe...

Plasmid incompatibility.

A bacterial plasmid is a species of nonessential extrachromosomal deoxyribonucleic acid (DNA) that replicates autonomously as a stable component of the cell's genome. Naturally occurring plasmids range in size from one to several hundred kilobases and in copy number from one to several hundred per cell. Copy number is a fixed characteristic of any plasmid under constant conditions and is contro...